Dr. Gylys' lab uses postmortem Alzheimer's tissue and transgenic mouse models of this disease to understand the mechanisms by which synapses degenerate in Alzheimer's. Current studies are focused on the contribution of apoE and lipids to the accumulation of amyloid-beta in synaptic terminals, and the role that this accumulation plays in early cognitive loss and lesion formation.
Apolipoprotein E level and cholesterol are associated with reduced synaptic amyloid beta in Alzheimer's disease and apoE TR mouse cortex. Arold S, Sullivan P, Bilousova T, Teng E, Miller CA, Poon WW, Vinters HV, Cornwell LB, Saing T, Cole GM, Gylys KH. Acta Neuropathol. 2011 Oct 22. PMID: 22020632
Preferential accumulation of amyloid-beta in presynaptic glutamatergic terminals (VGluT1 and VGluT2) in Alzheimer's disease cortex. Sokolow S, Luu SH, Nandy K, Miller CA, Vinters HV, Poon WW, Gylys KH. Neurobiol Dis. 2012 Jan;45(1):381-7. Epub 2011 Sep 3. PMID: 21914482
AD synapses contain abundant Aβ monomer and multiple soluble oligomers, including a 56-kDa assembly. Sokolow S, Henkins KM, Bilousova T, Miller CA, Vinters HV, Poon W, Cole GM, Gylys KH. Neurobiol Aging. 2011 Jul 6. PMID: 21741125
High levels of synaptosomal Na(+)-Ca(2+) exchangers (NCX1, NCX2, NCX3) co-localized with amyloid-beta in human cerebral cortex affected by Alzheimer's disease. Sokolow S, Luu SH, Headley AJ, Hanson AY, Kim T, Miller CA, Vinters HV, Gylys KH. Cell Calcium. 2011 Apr;49(4):208-16. Epub 2011 Mar 5. PMID: 21382638
Biochemical, neuropathological, and neuroimaging characteristics of early-onset Alzheimer's disease due to a novel PSEN1 mutation. Ringman JM, Gylys KH, Medina LD, Fox M, Kepe V, Flores DL, Apostolova LG, Barrio JR, Small G, Silverman DH, Siu E, Cederbaum S, Hecimovic S, Malnar M, Chakraverty S, Goate AM, Bird TD, Leverenz JB. Neurosci Lett. 2011 Jan 10;487(3):287-92. Epub 2010 Nov 19. Erratum in: Neurosci Lett. 2011 Mar 17;491(2):163. PMID: 21094210